Download PDF by Chirag Mandavia: Characterization of the Angiotensin receptor AT2 by various

By Chirag Mandavia

ISBN-10: 3659348147

ISBN-13: 9783659348143

book Date: February 22, 2013 | ISBN-10: 3659348147 | ISBN-13: 978-3659348143
In order to check AT2-mediated sign transduction mechanisms, major ways have been used: (1) the ligand-binding homes of mutant AT2 receptors have been characterised compared to wild-type after iteration of some degree mutation utilizing site-directed mutagenesis, and (2) interacting protein companions have been analyzed utilizing yeast library screening, co-immunoprecipitation and Western Blot experiences. We chanced on that the facility of Angiotensin II (Ang II) receptor sort AT2 Phe308Ala residue mutant within the seventh TMD to bind peptidic ligands was once significantly diminished, utilizing Xenopus oocytes expression procedure. this deposit occupies a place within the AT2 analogous to the Tyr292 within the AT1 crucial for agonistic activation of that receptor. Screening of a 17-day mouse embryo cDNA library with the Angiotensin II receptor AT2 because the bait in yeast two-hybrid assay pointed out sodium-hydrogen exchanger NHE6 because the binding associate with ninety eight% series homology. extra reviews printed that after human cell-line MCF-7 used to be transfected with the AT2, the AT2 co-immunoprecipitated with the NHE6 within the presence of Ang II, implying that Ang II-mediated activation is required for the interplay among AT2 and NHE6.
Show extra
Show less

Show description

Read Online or Download Characterization of the Angiotensin receptor AT2 by various studies PDF

Similar medical research books

Textbook of Research Ethics: Theory and Practice - download pdf or read online

This textbook offers a quick historical past of human experimentation and experiences numerous theories of ethics from which the rules and ideas that govern this study are derived. All appropriate foreign records and nationwide rules, rules and memoranda are stated largely to help in addressing matters that often come up through the process study concerning human matters.

Beth Dawson,Robert G. Trapp's Basic & Clinical Biostatistics 4/E (EBOOK) (LANGE Basic PDF

The appropriate strategy to advance sound judgment approximately info appropriate to scientific care First collection of scholars, educators, and practitioners a radical, significant, and fascinating presentation of biostatistics is helping scholars develop into proficient clients and shoppers of biostatistics discover ways to assessment and practice data in drugs, scientific learn, and all health-related fields.

Get The Laboratory Mouse (Handbook of Experimental Animals) PDF

Between animals utilized in learn, instructing and checking out, mice at the moment are widely known because the most vital version for human ailments and issues. They include the vast majority of all experimental mammals and have a tendency to be the version of selection used for study into many diseases/disorders together with melanoma, middle affliction, bronchial asthma, Alzheimer’s, Down syndrome, deafness, osteoporosis, weight problems, diabetes or even psychological future health examine.

Get Permutation Statistical Methods: An Integrated Approach PDF

This researchmonograph offers a synthesis of a couple of statistical assessments and measures,which, at the beginning attention, look disjoint and unrelated. Numerouscomparisons of permutation and classical statistical tools are awarded, andthe tools are in comparison through likelihood values and, the place appropriate,measures of impression dimension.

Additional resources for Characterization of the Angiotensin receptor AT2 by various studies

Sample text

Download PDF sample

Characterization of the Angiotensin receptor AT2 by various studies by Chirag Mandavia


by Kevin
4.3

Rated 4.27 of 5 – based on 18 votes

Published by admin